gut bacterial species  (ATCC)

Journal: BMC Microbiology

Article Title: Plasmid pHXY0908 confers ciprofloxacin heteroresistance to Salmonella enterica serovar typhimurium ATCC 14028 by regulating efflux pump gene expression

doi: 10.1186/s12866-025-04386-6

Figure Lengend Snippet: Chromosome CID region map of ATCC 14028 and ATCC 14028-pHXY0908 at 20Kb resolution. A Chromosome CID region map of ATCC 14028. B Chromosome CID region map of ATCC 14028-pHXY0908. Length: the length of a bacterial chromosome; the color bars represent the frequency of DNA fragments interact

Article Snippet: To assess whether plasmid pHXY0908 confers ciprofloxacin heteroresistance across bacterial species, we transformed the plasmid into standard strains of Salmonella enterica SL1344, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603, respectively.

Techniques:

Journal: BMC Microbiology

Article Title: Plasmid pHXY0908 confers ciprofloxacin heteroresistance to Salmonella enterica serovar typhimurium ATCC 14028 by regulating efflux pump gene expression

doi: 10.1186/s12866-025-04386-6

Figure Lengend Snippet: Population analysis assays A Population analysis assays of the Salomella Typhimurium SL1344-pHXY0908 strains using ciprofloxacin. B Population analysis assays of the Klebsiella pneumoniae ATCC 700,603-pHXY0908 strains using ciprofloxacin. C Population analysis assays of the Escherichia coli ATCC 25,922-pHXY0908 strains using ciprofloxacin

Article Snippet: To assess whether plasmid pHXY0908 confers ciprofloxacin heteroresistance across bacterial species, we transformed the plasmid into standard strains of Salmonella enterica SL1344, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603, respectively.

Techniques:

Journal: mBio

Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

doi: 10.1128/mbio.01099-25

Figure Lengend Snippet: Cytokine production of PBMCs incubated with K. pneumoniae previously exposed to Mars-like conditions. PBMC innate ( A ) and adaptive ( B ) cytokine responses. n = 9 biological replicates pooled from three independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. M9, minimal media; Unst, unstimulated; UV, ultraviolet.

Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Incubation, Comparison

Journal: mBio

Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

doi: 10.1128/mbio.01099-25

Figure Lengend Snippet: Effector functions of PBMCs against bacteria exposed to Mars-like conditions. Reactive oxygen species (ROS) production by PBMCs stimulated with K. pneumoniae ( A ) or S. marcescens ( B ). Percentage of CD11b+ cells positive for DTAF (FITC)-labeled bacteria ( C and E ) and representative histograms ( D and F ) for K. pneumoniae and S. marcescens . n = 6–9 biological replicates from two to three independent experiments, * P ≤ 0.05, ** P ≤ 0.01, **** P ≤ 0.0001 Friedman test followed by Dunn’s multiple comparison test. AUC, area under curve as a ratio of ROS production over time; M9, minimal media; Unst, unstimulated; UV, ultraviolet.

Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Bacteria, Labeling, Comparison

Journal: mBio

Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

doi: 10.1128/mbio.01099-25

Figure Lengend Snippet: Regrowth after desiccation partially rescues the effects of desiccation. Cytokine production following stimulation of PBMCs for 24 h ( A and E ) and 7 days ( B and F ) with K. pneumoniae ( A and B ) or S. marcescens ( E and F ). Reactive oxygen species production when stimulated with K. pneumoniae ( C ) or S. marcescens ( G ). Phagocytosis of K. pneumoniae ( D ) and S. marcescens ( H ) by CD11b+ cells. n = 6 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test with Dunn’s multiple comparison test. M9, minimal media.

Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Comparison

Journal: mBio

Article Title: Effects of simulated Martian environmental stressors on specific human pathogen–immune system interactions

doi: 10.1128/mbio.01099-25

Figure Lengend Snippet: The morphology of bacterial cells is altered following exposure to simulated Martian conditions. TEM visualization of K. pneumoniae ( A ) and S. marcescens ( C ), size (FSC) and complexity (SSC) quantification by flow cytometry of K. pneumoniae ( B ) and S. marcescens ( D ). Scale bars are 500 nm in panel A and 250 nm in panel C. n = 5–7 biological replicates from two independent experiments. * P ≤ 0.05, ** P ≤ 0.01. Friedman test followed by Dunn’s multiple comparison test. FSC, forward scatter; M9, minimal media; SSC, side scatter; UV, ultraviolet.

Article Snippet: The bacterial species K. pneumoniae 298-53 (DSM 30104) and S. marcescens BS303 (DSM 30121) were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH.

Techniques: Flow Cytometry, Comparison